![]() The temporary hyperglycemia was 11 ± 1 versus 19 ± 5 days (p40-50% following T-cell replete reduced intensity transplantation correlates with a high risk of graft rejection, regardless of donor-lymphocyte infusions, but it is unclear whether this finding translates to T-cell depleted transplants. SRDX SEIKO FULLFull but not low-grade or no donor chimerism was associated with successful engraftment of allogeneic islets and restoration of normoglycemia in the treated diabetic mice. We also studied the anti-inflammatory effect of mesenchymal stem cell on islet engraftment. ![]() We used bone marrow stem cells from C57BL/6 (H2b mice to construct donor chimerism in conditioned diabetic BALB/c (H2d mice and investigated the effect of donor chimerism on engraftment and survival of subcutaneously transplanted allogeneic islets in streptozotocin-induced diabetic mice. Prolonged Survival of Subcutaneous Allogeneic Islet Graft by Donor Chimerism without Immunosuppressive Treatmentĭirectory of Open Access Journals (Sweden)įull Text Available The aim of this study was to investigate whether tolerance-induced protection of islets in the renal subcapsular space can also prevent subcutaneous allogeneic islets from being rejected. Reprint & Copyright © 2014 Association of Military Surgeons of the U.S. SRDX SEIKO SKINThese feasibility studies established the proof-of-principle necessary to further develop chimeric autologous/ allogeneic constructs for the treatment of complex skin defects. ![]() Our results confirmed successful MTC insertion and integration, and demonstrated the feasibility of generating chimeric autologous/ allogeneic constructs that preserved the viability, proliferative capacity, and structure of autologous pigmented skin. Tissue viability, proliferative capacity, and morphology were evaluated after long-term culture. After MTC insertion, chimeric constructs and controls were reintroduced into air-interface culture and maintained in vitro for several weeks. Chimeric constructs have the potential to provide immediate wound coverage, eliminate painful donor site wounds, and promote restoration of a pigmented skin tissue possessing hair follicles, sweat glands, and sebaceous glands. We evaluated the feasibility of combining these two technologies by embedding MTCs in NIKS-based skin tissues to generate chimeric autologous/ allogeneic constructs. Independently, a device has recently been created to "copy" skin by harvesting full-thickness microscopic tissue columns (MTCs) in lieu of autografts traditionally harvested as sheets. NIKS progenitor cell-based skin tissues have been developed to promote healing by providing barrier function and delivering wound healing factors. The ideal treatment for severe cutaneous injuries would eliminate the need for autografts and promote fully functional, aesthetically pleasing autologous skin regeneration. Rasmussen, Cathy Ann Tam, Joshua Steiglitz, Barry M Bauer, Rebecca L Peters, Noel R Wang, Ying Anderson, R Rox Allen-Hoffmann, B Lynn ![]() Chimeric autologous/ allogeneic constructs for skin regeneration. ![]()
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